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dc.contributor.authorCushnie, T. P. Tim
dc.contributor.authorO'Driscoll, Noelle H.
dc.contributor.authorLamb, Andrew
dc.date.accessioned2017-01-24T12:02:48Z
dc.date.available2017-01-24T12:02:48Z
dc.date.issued2016-07-28en
dc.identifier.citationCUSHNIE, T.P.T., O'DRISCOLL, N.H. and LAMB, A.J. 2016. Morphological and ultrastructural changes in bacterial cells as an indicator of antibacterial mechanism of action. Cellular and molecular life sciences [online], 73(23), pages 4471-4492. Available from: https://doi.org/10.1007/s00018-016-2302-2en
dc.identifier.issn1420-682Xen
dc.identifier.issn1420-9071en
dc.identifier.urihttp://hdl.handle.net/10059/2129
dc.description.abstractEfforts to reduce the global burden of bacterial disease and contend with escalating bacterial resistance are spurring innovation in antibacterial drug and biocide development and related technologies such as photodynamic therapy and photochemical disinfection. Elucidation of the mechanism of action of these new agents and processes can greatly facilitate their development, but it is a complex endeavour. One strategy that has been popular for many years, and which is garnering increasing interest due to recent technological advances in microscopy and a deeper understanding of the molecular events involved, is the examination of treated bacteria for changes to their morphology and ultrastructure. In this review, we take a critical look at this approach. Variables affecting antibacterial-induced alterations are discussed first. These include characteristics of the test organism (e.g. cell wall structure) and incubation conditions (e.g. growth medium osmolarity). The main body of the review then describes the different alterations that can occur. Micrographs depicting these alterations are presented, together with information on agents that induce the change, and the sequence of molecular events that lead to the change. We close by highlighting those morphological and ultrastructural changes which are consistently induced by agents sharing the same mechanism (e.g. spheroplast formation by peptidoglycan synthesis inhibitors) and explaining how changes that are induced by multiple antibacterial classes (e.g. filamentation by DNA synthesis inhibitors, FtsZ disruptors, and other types of agent) can still yield useful mechanistic information. Lastly, recommendations are made regarding future study design and execution.en
dc.language.isoengen
dc.publisherSpringeren
dc.rightshttps://creativecommons.org/licenses/by-nc-nd/4.0en
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectAntibioticen
dc.subjectAntisepticen
dc.subjectDisinfectanten
dc.subjectMode of actionen
dc.subjectSEMen
dc.subjectTEMen
dc.titleMorphological and ultrastructural changes in bacterial cells as an indicator of antibacterial mechanism of action.en
dc.typeJournal articlesen
dc.publisher.urihttps://doi.org/10.1007/s00018-016-2302-2en
dcterms.dateAccepted2016-06-28en
dcterms.publicationdate2016-12-31en
refterms.accessExceptionNAen
refterms.dateDeposit2017-01-24en
refterms.dateEmbargoEnd2017-12-31en
refterms.dateFCA2017-12-31en
refterms.dateFCD2017-01-24en
refterms.dateFreeToDownload2017-12-31en
refterms.dateFreeToRead2017-12-31en
refterms.dateToSearch2017-12-31en
refterms.depositExceptiondelaySecuringTexten
refterms.panelAen
refterms.technicalExceptionNAen
refterms.versionAMen
rioxxterms.publicationdate2016-07-08en
rioxxterms.typeJournal Article/Reviewen
rioxxterms.versionAMen


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