Use of a three dimensional porcine retinal explant model to detect HIF1 alpha for understanding diabetic retinopathy.
Cruickshank, Stuart F.
Knott, Rachel M.
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IWUAGWU, B.U., CRUICKSHANK, S.F., ROWE, I. and KNOTT, R.M. 2017. Use of a three dimensional porcine retinal explant model to detect HIF1 alpha for understanding diabetic retinopathy. Presented at the American Society for Biochemistry and Molecular Biology annual meeting (ASBMB 2017), 22-26 April 2017, Chicaco, USA.
Diabeticretinopathy (DR) is a major microvascular complication associated with diabetes mellitus and is a leading cause of blindness. Hypoxia and hyperglycemia are suggested to play crucial pathophysiological roles, but the exact mechanisms are complex and the precise events are still debated. Models of DR, including organotypic cultures (retinal explants) contribute significantly to studies in DR. Here, a retinal explant model which comprised of whole porcine retina embedded within an agarose-collagen three-dimension (3D) matrix is utilised. Explants were cultured in 5.5mM (low) or 25mM (high) glucose for up to 24 h. The aim of this work is to investigate the expression and localization of the hypoxia inducible factor -1 alpha (HIF1α),a critical transcription factor in DR, and other targets:endothelial proliferation marker (CD31), glial fibrillary acidic protein (GFAP) and cellular proliferation marker (Ki67) in explants.