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dc.contributor.authorMitchell, Catriona J.
dc.contributor.authorO'Sullivan, Catherine M.
dc.contributor.authorPinloche, Eric
dc.contributor.authorWilkinson, Toby
dc.contributor.authorMorphew, Russell M.
dc.contributor.authorMcEwan, Neil R.
dc.date.accessioned2019-04-19T09:37:53Z
dc.date.available2019-04-19T09:37:53Z
dc.date.issued2019-04-03en
dc.identifier.citationMITCHELL, C.J., O'SULLIVAN, C.M., PINLOCHE, E., WILKINSON, T., MORPHEW, R.M. and MCEWAN, N.R. 2019. Using next generation sequencing to determine diversity of horse intestinal worms: identifying the equine 'nemabiome'. Journal of equine science [online], 30(1), pages 1-5. Available from: https://doi.org/10.1294/jes.30.1en
dc.identifier.issn1340-3516en
dc.identifier.issn1347-7501en
dc.identifier.urihttp://hdl.handle.net/10059/3375
dc.description.abstractNext generation sequencing of DNA from nematode eggs has been utilised to give the first account of the equine ‘nemabiome’. In all equine faecal samples investigated, multiple species of Strongylidae were detected; ranging from 7.5 (SEM 0.79) with 99+% identity to sequences in the NCBI database to 13.3 (SEM 0.80) with 90+% identity. This range is typical of the number of species described previously in morphological studies using large quantities of digesta per animal. However, the current method is non-invasive, relies on DNA analysis avoiding the need for specialist microscopy identification and can be carried out with small samples providing significant advantages over current methods.en
dc.description.sponsorshipESF European Social Fund (Access to Masters Scholarship).en
dc.language.isoengen
dc.publisherJapanese Society of Equine Scienceen
dc.rightshttps://creativecommons.org/licenses/by-nc-nd/4.0en
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectHorseen
dc.subjectFaecal eggsen
dc.subjectNemabiomeen
dc.subjectDiversityen
dc.titleUsing next-generation sequencing to determine diversity of horse intestinal worms: identifying the equine ‘nemabiome’.en
dc.typeJournal articlesen
dc.publisher.urihttps://doi.org/10.1294/jes.30.1en
dcterms.dateAccepted2019-02-14en
dcterms.publicationdate2019-03-31en
refterms.accessExceptionNAen
refterms.dateDeposit2019-04-19en
refterms.dateFCA2019-04-19en
refterms.dateFCD2019-04-19en
refterms.dateFreeToDownload2019-04-19en
refterms.dateFreeToRead2019-04-19en
refterms.dateToSearch2019-04-19en
refterms.depositExceptionNAen
refterms.panelAen
refterms.technicalExceptionNAen
refterms.versionVoRen
rioxxterms.publicationdate2019-04-03en
rioxxterms.typeJournal Article/Reviewen
rioxxterms.versionVoRen


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