OpenAIR @ RGU >
Health and Social Care >
Pharmacy & Life Sciences >
Theses (Pharmacy & Life Sciences) >
Please use this identifier to cite or link to this item:
|Title: ||Degradation of microcystin-LR by aquatic bacteria|
|Authors: ||Ghimire, Buddhi Sagar|
|Supervisors: ||Lawton, Linda A.|
Biolog MT2 plate
|Issue Date: ||Jun-2007|
|Publisher: ||The Robert Gordon University|
|Abstract: ||Microcystins are cyclic heptapeptide toxins produced by cyanobacteria. They
are potentially a threat to human and domestic animals so are of worldwide
interest. Microcystin-LR is stable in various environmental conditions. It is
important to know the factors affecting microcystin degradation which can
be utilised in water treatment methods. This research is focused on activity
of indigenous microflora in biolog MT2 plates and use of active isolates for
degradation study. The growth and degradation patterns were compared
with the known microcystin-LR degrading bacteria Paucibacter toxinivorans
(DSMZ-16998) as a positive control. A total of 18 bacteria were isolated
from three different sources and screened for the degradation study.
Individual isolates and P. toxinivorans were exposed to microcystin-LR in a
range of different media namely, physiological saline, R2A medium, nutrient
broth and water at different concentrations of 0.50 1g/ml and 10.00 1g/ml
of microcystin-LR. Degradation of microcystin-LR content was analysed by
HPLC. Bacteria in mixed culture i.e. natural water samples were able to
degrade microcystin-LR in low concentration within a week but were unable
to degrade in high concentration until 30 days at room temperature.
Degradation of microcystin-LR by cell extract of active isolates and P.
toxinivorans was observed. Percentage loss of microcystin content by
enzymatic degradation was observed from 22-42 percent in tested samples.|
|Appears in Collections:||Theses (Pharmacy & Life Sciences)|
All items in OpenAIR are protected by copyright, with all rights reserved.